PsychoProt: Physical CHemistry Of Protein variability

Concept art: How protein traits emerge from amino acid properties

A website for the analysis of protein sequence variability in terms of physicochemical descriptors of the amino acids.

For a given dataset (from alignments, deep-sequencing experiments, etc.) the main tool will find out which property(ies) of the amino acids (volume, hydrophobicity, etc.) shape the probability of finding each amino acid at each position of the sequence. Such kind of analysis helps retrieve rich information about the main physicochemical constraints to protein design, engineering and evolution, as illustrated in this application to deep-sequencing data on a library of all mutants at all positions of an enzyme.